Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Comparison of the expression of 2 representative biochemical markers in both eyes of dry eye patients ( N = 23). ( a ) concentration of tear matrix metallopeptidase 9 (MMP-9) ( p = 0.9881); ( b ) concentration of tear lactoferrin ( p > 0.9999); and ( c ) concentration ratio of MMP-9 to lactoferrin of each tear sample ( p = 0.7540). ( d ) correlation of tear MMP-9 concentration; ( e ) correlation of tear lactoferrin concentration; ( f ) correlation of concentration ratio of MMP-9 to lactoferrin of binocular tear samples of each patients. Abbreviation: LF, lactoferrin.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: Comparison, Expressing, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Comparison of lactoferrin-corrected standardized signals for six always presented molecules in binocular tear samples of dry eye subjects ( N = 23). ( a ) lactoferrin ( p = 0.8422); ( b ) lipocalin-1 ( p = 0.6221); ( c ) lysozyme C ( p = 0.9168); ( d ) Immunoglobulin alpha-1 chain C region ( p = 0.4820); ( e ) lacritin ( p = 0.5803); and ( f ) prolactin-inducible protein ( p = 0.3604). Abbreviation: Ig, immunoglobulin.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: Comparison

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Binocular association of lactoferrin-corrected standardized signals for six always presented molecules in tear samples of dry eye patients. ( a ) lactoferrin; ( b ) lipocalin-1; ( c ) lysozyme C ( d ) immunoglobulin alpha-1 chain C region; ( e ) lacritin; and ( f ) prolactin-inducible protein. Abbreviation: Ig, immunoglobulin.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Association between representative ocular surface homeostatic markers and tear biomarkers in the ipsilateral eye. ( a ) right eye ( N = 23); ( b ) left eye ( N = 23). Abbreviation: OSDI, ocular surface disease index; TMH_c, central tear meniscus height; BR_t, temporal bulbar redness score; NIKBUT_f, non-invasive keratographic first break-up time; NIKBUT_at, assessable time of non-invasive keratographic break-up test; Oxford, Oxford staining score; MeS, meiboscale; LF, lactoferrin; IGHA1, immunoglobulin alpha-1 chain C region; and PIP, prolactin-inducible protein.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study?

doi: 10.3390/ijms22010422

Figure Lengend Snippet: Association between ocular surface homeostasis and tear biomarkers in the ipsilateral eye.

Article Snippet: The lactoferrin concentrations of all tear samples were measured using a human lactoferrin enzyme-linked immunosorbent assay (ELISA) kit (Catalog no. KA0484, Abnova, Taipei, Taiwan).

Techniques:

Journal: Diagnostics

Article Title: Poor Performance of Angiotensin II Enzyme-Linked Immuno-Sorbent Assays in Mostly Hypertensive Cohort Routinely Screened for Primary Aldosteronism

doi: 10.3390/diagnostics12051124

Figure Lengend Snippet: The study protocol. The study cohort included the population referred to screening for primary aldosteronism. Of all antihypertensives, only MRAs were excluded before the first screening test. The participants underwent hormonal evaluation of angiotensin II, aldosterone, and DRC. ARR and AA2R were calculated. After the first screening test, the patients were properly prepared and further diagnosed until the final diagnosis which revealed 20 cases of PA.

Article Snippet: Pre-stored frozen plasma was used to determine angiotensin II manually in a local hospital’s department of radioimmunology with a Human Ang II ELISA Kit (FineTest, Biotech Co., Ltd., Wuhan, China), with no previous chromatographic separation.

Techniques: Biomarker Discovery

Journal: Diagnostics

Article Title: Poor Performance of Angiotensin II Enzyme-Linked Immuno-Sorbent Assays in Mostly Hypertensive Cohort Routinely Screened for Primary Aldosteronism

doi: 10.3390/diagnostics12051124

Figure Lengend Snippet: Clinical and biochemical characteristics of the study cohort—comparison between females and males.

Article Snippet: Pre-stored frozen plasma was used to determine angiotensin II manually in a local hospital’s department of radioimmunology with a Human Ang II ELISA Kit (FineTest, Biotech Co., Ltd., Wuhan, China), with no previous chromatographic separation.

Techniques:

Journal: Diagnostics

Article Title: Poor Performance of Angiotensin II Enzyme-Linked Immuno-Sorbent Assays in Mostly Hypertensive Cohort Routinely Screened for Primary Aldosteronism

doi: 10.3390/diagnostics12051124

Figure Lengend Snippet: ( a ) Angiotensin II levels were not statistically different between PA ( n = 20) and EH groups ( n = 80) (773.5 vs. 873.2 pg/mL, p = 0.23); ( b – d ) the correlation between Ang II and DRC: ( b ) in the whole study population, with Spearman’s rho = 0.20 ( p = 0.04), ( c ) in the patients receiving other drugs than ACEI (64 participants) with Spearman’s rho = 0.23 ( p = 0.07), and ( d ) in the EH group (non-PA, 80 patients), with Spearman’s rho = 0.18 ( p = 0.12).

Article Snippet: Pre-stored frozen plasma was used to determine angiotensin II manually in a local hospital’s department of radioimmunology with a Human Ang II ELISA Kit (FineTest, Biotech Co., Ltd., Wuhan, China), with no previous chromatographic separation.

Techniques:

Journal: Diagnostics

Article Title: Poor Performance of Angiotensin II Enzyme-Linked Immuno-Sorbent Assays in Mostly Hypertensive Cohort Routinely Screened for Primary Aldosteronism

doi: 10.3390/diagnostics12051124

Figure Lengend Snippet: Hormonal results—comparison between PA and EH groups.

Article Snippet: Pre-stored frozen plasma was used to determine angiotensin II manually in a local hospital’s department of radioimmunology with a Human Ang II ELISA Kit (FineTest, Biotech Co., Ltd., Wuhan, China), with no previous chromatographic separation.

Techniques: Clinical Proteomics

Journal: Diagnostics

Article Title: Poor Performance of Angiotensin II Enzyme-Linked Immuno-Sorbent Assays in Mostly Hypertensive Cohort Routinely Screened for Primary Aldosteronism

doi: 10.3390/diagnostics12051124

Figure Lengend Snippet: ROCs for ( a ) angiotensin II and ( b ) AA2R; ( c ) comparison of ROCs for ADRR I, AA2R, aldosterone, DRC, and hypokalemia (all p < 0.01).

Article Snippet: Pre-stored frozen plasma was used to determine angiotensin II manually in a local hospital’s department of radioimmunology with a Human Ang II ELISA Kit (FineTest, Biotech Co., Ltd., Wuhan, China), with no previous chromatographic separation.

Techniques: Comparison